Cat preantral follicle survival after prolonged cooled storage followed by vitrification

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In vivo and in vitro strategies to support caprine preantral follicle development after ovarian tissue vitrification.

The aim of the present study was to compare fresh and vitrified goat ovarian tissue after autotransplantation and in vitro culture. Adult goats were completely ovariectomised and each ovarian pair was sliced and distributed among six different treatment groups: fresh control, fresh transplant, fresh culture, vitrified control, vitrified transplant and vitrified culture. Follicular morphology, d...

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Myocardial hydroxyproline and mechanical response to prolonged pressure loading followed by unloading in the cat.

To determine the myocardial response to prolonged pressure-loading and unloading, kittens weighing 0.8-1.2 kg underwent pulmonary artery banding, which initially elevated right ventricular (RV) systolic pressure by 10-15 mm Hg. 52 and 76 wk later; RV weight/body weight had increased by approximately 80%. Total RV hydroxyproline had increased significantly, whereas hydroxyproline concentration w...

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Progesterone and Follicle Stimulating Hormone interact and promote goat preantral follicles survival and development in vitro1

Lima-Verde I.B., Matos M.H.T., Celestino J.J.H., Rossetto R., Name K.P.O., Báo S.N., Campello C.C. & Figueiredo J.R. 2012. Progesterone and Follicle Stimulating Hormone interact and promote goat preantral follicles survival and development in vitro. Pesquisa Veterinária Brasileira 32(4):361-367. Programa de Pós-Graduação em Ciências Veterinárias, Laboratório de Manipulação de Oócitos e Folículo...

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P-83: Development of Mouse Preantral Follicles in Fibrin-Alginate Matrix during In Vitro Culture

Background This research was conducted to assess preantral follicle development in fibrin alginate matrix following ovarian tissue vitrification MaterialsAndMethods Ovaries of 13-day-old NMRI female mice were removed and placed in control and vitrification groups. Vitrification group ovaries were transferred in media containing ethylene glycol, dimethyl sulphoxide, and sucrose then were plunged...

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Development of Mouse Preantral Follicle after In Vitro Culture in A Medium Containing Melatonin

OBJECTIVE Improvements in cancer treatment have allowed more young women to survive. However, many cancer patients suffer from ovarian failure. Cryopreservation is one of the solutions for fertility restoration in these patients. The cryopreservation of isolated follicles is a more attractive approach in the long term. Many endocrine and paracrine factors can stimulate the granulosa cells of pr...

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ژورنال

عنوان ژورنال: Cryobiology

سال: 2018

ISSN: 0011-2240

DOI: 10.1016/j.cryobiol.2018.02.004